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btr [2018-05-18 16:53:27]
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btr [2018-05-18 16:53:27]

transcriptional activator (AraC family), (AraC family DNA-binding domain fused to FeuA-like substrate-binding domain), regulation of the feuA-feuB-feuC-ybbA operon
locus
BSU01640
pI
6.43
mw
60.59 kDa
protein length
529 aa Sequence Blast
gene length
1587 bp Sequence Blast
function
regulation of iron acquisition
product
transcription activator (AraC family)
essential
no
synonyms
ybbB, yzbC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    183,414 → 185,003

    The protein

    Catalyzed reaction/ biological activity

  • binding of a direct repeat in the ''[gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]'' promoter region and transcription activation of the ''[gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA]'' operon in the presence of bacillibactin or enterobactin [Pubmed|17725565,22210890]

    • Protein family

  • [SW|AraC family]

    • [SW|Cofactors]

  • enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

    • Effectors of protein activity

  • enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

    • Expression and Regulation

    Operons

    genes
    [gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]
    description
    [Pubmed|12354229]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • expressed under conditions of iron starvation ([protein|search|Fur]) [Pubmed|12354229]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • MGNA-B949 (ybbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1948 NBRP B. subtilis, Japan]
  • BKE01640 (Δ[gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC, downstream forward: _UP4_TGATAAATCAGGAATTTGTC
  • BKK01640 (Δ[gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC, downstream forward: _UP4_TGATAAATCAGGAATTTGTC

    • References

  • 12884008,17725565,22210890,12354229,23199363,23504016